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Proteomics Profiling of Single Organs from Individual Adult Zebrafish.

Journal article
Authors Alexandra Abramsson
Ann Brinkmalm-Westman
Josef Pannee
Mikael K Gustavsson
Malin von Otter
Kaj Blennow
Gunnar Brinkmalm
Petronella Kettunen
Henrik Zetterberg
Published in Zebrafish
Volume 7
Issue 2
Pages 161-168
ISSN 1557-8542
Publication year 2010
Published at Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Pages 161-168
Language en
Links dx.doi.org/10.1089/zeb.2009.0644
Subject categories Psychiatry

Abstract

Abstract The model organism zebrafish (Danio rerio) is extensively utilized in studies of developmental biology but is also being investigated in the context of a growing list of human age-related diseases. To facilitate such studies, we here present protein expression patterns of adult zebrafish organs, including blood, brain, fin, heart, intestine, liver, and skeletal muscle. Protein extracts were prepared from the different organs of two zebrafish and analyzed using liquid chromatography coupled to high-resolution tandem mass spectrometry. Zebrafish tissue was digested directly after minimal fractionation and cleaned up (the shotgun approach). Proteins were identified using Mascot software. In total, 1394 proteins were identified of which 644 were nonredundant. Of these, 373 demonstrated an organ-specific expression pattern and 57 had not been shown on protein level before. These data emphasize the need for increased research at the protein level to facilitate the selection of candidate proteins for targeted quantification and to refine systematic genetic network analysis in vertebrate development, biology, and disease.

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