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Variations in coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein film during adsorption and cross-linking: a quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study

Journal article
Authors F. Hook
B. Kasemo
T. Nylander
C. Fant
Kristin Sott
Hans-Björne Elwing
Published in Analytical Chemistry
Volume 73
Issue 24
Pages 5796-804
ISSN 0003-2700
Publication year 2001
Published at Department of Cell and Molecular Biology
Department of Physics (GU)
Pages 5796-804
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Adsorption, Algorithms, Animals, Bivalvia/chemistry, Cross-Linking Reagents, Elasticity, Kinetics, Nanotechnology, Proteins/*chemistry, Surface Plasmon Resonance, Viscosity, Water/chemistry
Subject categories Chemical Sciences

Abstract

We have measured the time-resolved adsorption kinetics of the mussel adhesive protein (Mefp-1) on a nonpolar, methyl-terminated (thiolated) gold surface, using three independent techniques: quartz crystal microbalance with dissipation monitoring (QCM-D), surface plasmon resonance, and ellipsometry. The QCM-D and ellipsometry data shows that, after adsorption to saturation of Mefp-1, cross-linking of the protein layer using NaIO4 transforms it from an extended (approximately 20 nm), water-rich, and hydrogel-like state to a much thinner (approximately 5 nm), compact, and less water-rich state. Furthermore, we show how quantitative data about the thickness, shear elastic modulus, and shear viscosity of the protein film can be obtained with the QCM-D technique, even beyond the Sauerbrey regime, if frequency (f) and energy dissipation (D) measurements measured at multiple harmonics are combined with theoretical simulations using a Voight-based viscoelastic model. The modeling result was confirmed by substituting H2O for D2O. As expected, the D2O substitution does not influence the actual adsorption behavior, but resulted in expected differences in the estimated effective density and shear viscosity. These results provide new insight and understanding about the adsorption kinetics and crosslinking behavior of Mefp-1. They also demonstrate how the above three techniques complement each other for biomolecule adsorption studies.

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