To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

A micro-fluidic system fo… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

A micro-fluidic system for studies of stress response in single cells using optical tweezers

Conference paper
Authors Annette Graneli
Emma Eriksson
Jonas Enger
Kerstin Ramser
Mattias Goksör
Stefan Hohmann
Dag Hanstorp
Published in SPIE Proceedings: Optical Trapping and Optical Micromanipulation III
Volume 6326
Pages U142-U150
Publication year 2006
Published at Department of Cell and Molecular Biology
Department of Physics (GU)
Pages U142-U150
Language en
Subject categories Biological physics

Abstract

In recent years there has been a growing interest in the use of optical manipulation techniques, such as opticaltweezers, in biological research as the full potential of such applications are being realized. Biological research is developing towards the study of single entities to reveal new behaviors that cannot be discovered with more traditional ensemble techniques. To be able to study single cells we have developed a new method where a combination of micro-fluidics and optical tweezers was used. Micro-fluidic channels were fabricated using soft lithography. The channels consisted of a Y-shaped junction were two channels merged into one. By flowing different media in the two channels in laminar flow we were able to create a sharp concentration gradient at the junction. Single cells were trapped by the tweezers and the micro-fluidic system allowed fast environmental changes to be made for the cell in a reversible manner. The time required to change the surroundings of the cell was limited to how sharp mixing region the system could create, thus how far the cells had to be moved using the optical tweezers. With this new technique cellular response in single cells upon fast environmental changes could be investigated in real time. The cellular response was detected by monitoring variations in the cell by following the localization of fluorescently tagged proteins within the cell.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?