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The DNA ligands influence the interactions between the herpes simplex virus 1 origin binding protein and the single strand DNA-binding protein, ICP-8.

Journal article
Authors Claes M Gustafsson
Maria Falkenberg
Stina Simonsson
Hadi Valadi
Per Elias
Published in The Journal of biological chemistry
Volume 270
Issue 32
Pages 19028-34
ISSN 0021-9258
Publication year 1995
Published at Institute of Medical Biochemistry
Pages 19028-34
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Base Sequence, Binding Sites, DNA Helicases, metabolism, DNA Replication, DNA, Single-Stranded, metabolism, DNA-Binding Proteins, metabolism, Ligands, Molecular Sequence Data, Viral Proteins, metabolism
Subject categories Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Abstract

The herpes simplex virus type 1 (HSV-1) origin binding protein, OBP, is a DNA helicase specifically stimulated by the viral single strand DNA-binding protein, ICP-8. The stimulation is dependent on direct protein-protein interactions between the C-terminal domain of OBP, delta OBP, and ICP 8 (Boehmer, P.E., Craigie, M.C., Stow, N.D., and Lehman, I.R. (1994) J. Biol. Chem. 269, 29329-29334). We have now observed that this interaction is dramatically influenced by the nature of the DNA ligand. Stable complexes between delta OBP, ICP 8, and double-stranded DNA, presented either as a specific duplex oligonucleotide or a restriction fragment containing the HSV-1 origin of replication, oriS, can be detected by gel chromatography and gel electrophoresis. In contrast, a single-stranded oligonucleotide, oligo(dT)65, will completely disrupt the complex between delta OBP and ICP 8. We therefore suggest that the interaction between delta OBP and ICP 8 serves to position the single strand DNA-binding protein with high precision onto single-stranded DNA at a replication fork or at an origin of DNA replication.

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