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Toxic activity of the CdtB component of Haemophilus ducreyi cytolethal distending toxin expressed from an adenovirus 5 vector

Journal article
Authors Catharina Wising
Maria K Magnusson
Karin Ahlman
Leif Lindholm
Teresa Lagergård
Published in APMIS
Volume 118
Issue 2
Pages 143–149
ISSN 1600-0463
Publication year 2010
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 143–149
Language en
Links dx.doi.org/10.1111/j.1600-0463.2009...
Keywords Haemophilus ducreyi, cytolethal distending toxin, adenovirus 5, cdtB insert
Subject categories Microbiology in the medical area

Abstract

The Haemophilus ducreyi cytolethal distending toxin (HdCDT) catalytic subunit CdtB has DNase-like activity and mediates DNA damage after its delivery into target cells. We constructed a replication-deficient adenovirus type 5 (Ad5) vector expressing CdtB and investigated the toxic properties of this vector on HeLa cells. Ad5CdtB caused loss of cell viability, morphologic changes, and cell cycle arrest, findings similar to HdCDT intoxication. This confirmed that CdtB is responsible for the toxicity of the holotoxin when expressed in cells following transduction by an adenoviral vector, and indicated a possible potential of this novel strategy in studies of activity of intracellular products and in gene therapy of cancer.

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