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Isolation and characterisation of a novel simazine-degrading bacterium from agricultural soil of central Chile, Pseudomonas nitroreducens MHP41

Poster
Authors Marcela Hernández
Veronica Vorgante
Christel Unosson
Edward R.B. Moore
Michael Seeger
Published in FEMS 2009 - 3rd Congress of European Microbiologists
Pages 150
Publication year 2009
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 150
Language en
Subject categories Microbiology

Abstract

Background: s-Triazine herbicides, such as simazine, are used in America in agriculture and forestry. This herbicide is persistent in soils, as well as in aquifers. Chemical and biological processes are involved in the dissipation of simazine in soil. However, microbial degradation is probably the main mechanism for removing s-triazine from soils. Objectives: 1. Isolation, metabolic and systematic characterization of bacterium MHP41 from herbicide-contaminated agricultural soil in Chile. Methods: Bacterial strains were isolated by enrichment in minimal medium with simazine as the sole nitrogen source. Simazine degradation was analysed by resting cell assays and analyses of atz genes were assessed by PCR and DNA sequencing. Strain MHP41 was characterised by ARDRA, using restriction endonucleases, MspI and HhaI, phenotypic characterisation, cell fatty acid profiling and multi-locus sequence analysis (MLSA), using 16S rRNA gene, IGS-1, gyrB and rpoB sequencing. Results: 1. Strain MHP41 grew in minimal medium, using simazine as the sole source of nitrogen. 2. Resting cells of strain MHP41 degraded more than 80% of simazine within 60 min. 3. The atzA, atzB, atzC, atzD, atzE and atzF degradation genes were detected by PCR and characterised by sequence analysis. 4. Strain MHP41 was identified initially as a Pseudomonas sp. and comparative MLSA determined MHP41 to be a strain of P. nitroreducens. Conclusions: 1. The isolation and characterization of a simazine-degrading bacterium MHP41, which is capable of growing with simazine as the sole nitrogen source. 2. P. nitroreducens MHP41 possesses all atz genes of the upper and lower catabolic pathways for simazine degradation. 4. MHP41 is the first known strain of P. nitroreducens capable of degrading simazine and represents an interesting bacterium for studies on the bioremediation of s-triazine-contaminated soils.

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