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Characterisation of endobacterial communities in ectomycorrhizas by DNA- and RNA-based molecular methods

Journal article
Authors H Izumi
Edward R.B. Moore
K Killham
IJ Alexander
IC Anderson
Published in Soil Biology and Biochemistry
Volume 39
Issue 4
Pages 891-899
Publication year 2007
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 891-899
Language en
Links www.sciencedirect.com/science?_ob=M...
Subject categories Microbiology

Abstract

The diversity of endobacteria associated with ectomycorrhizas of Suillus variegatus and Tomentellopsis submollis, in two Corsican pine (Pinus nigra) stands was analysed by cultivation-dependent and cultivation-independent molecular methods. Denaturing gradient gel electrophoresis (DGGE) analysis revealed the cultivable endobacterial communities associated with S. variegatus were similar within the same stand. The most abundant cultivable bacterial species belonged to the genera Pseudomonas and Burkholderia. Cultivation-independent molecular analysis indicated that the structure of the endobacterial communities in ectomycorrhizas was consistent across all samples regardless of ECM fungal species or the pine stand from which the samples were collected. However, comparison between rDNA- and rRNA-derived DGGE gels showed that metabolically active endobacterial species were not always detected in rDNA-based profiles. Clone libraries constructed from rRNA molecules indicated that Pseudomonas and Burkholderia spp. were metabolically active bacteria. As some of the most abundant cultivable bacteria, including Bacillus/Paenibacillus spp., were not detected in cultivation-independent DGGE profiles, a combination of cultivation-dependent and -independent approaches provided a more complete assessment of the diversity of endobacteria associated with ectomycorrhizas.

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