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Monitoring of the degradation activities and the diversity of the microbial community degrading refinery waste sludge

Journal article
Authors E Katsivela
Edward R.B. Moore
N Kalogerakis
Published in Water, Air & Soil Pollution: Focus
Volume 4
Issue 4
Pages 75-85
ISSN 1573-2940
Publication year 2004
Published at Institute of Laboratory Medicine, Dept of Clinical Bacteriology
Pages 75-85
Language en
Links www.springerlink.com/content/w08mj2...
Keywords bacterial community, biodegradation, petroleum hydrocarbons, refinery waste sludge, 16S rDNA, T-RFLP fingerprinting
Subject categories Morphology

Abstract

The biodegradation of n-alkanes and branched alkanes from waste sludge were observed in landfarming soils of Motor Oil Hellas (a petroleum refinery) and changes in the bacterial communities in the soils were monitored during the remediation. Bacterial 16S rRNA gene (rDNA)-based community fingerprint patterns were obtained from soil samples by terminal restriction fragment length polymorphism (T-RFLP) analysis. Changes in T-RFLP fingerprints, as well as in the petroleum contaminant composition of the polluted soil, correlated with degradation activities in field tests.

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