To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Bacterial community dynam… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites.

Journal article
Authors E Katsivela
Edward R.B. Moore
D Maroukli
C Strömpl
D Pieper
N Kalogerakis
Published in Biodegradation
Volume 16
Issue 2
Pages 169-80
ISSN 0923-9820
Publication year 2005
Published at Institute of Laboratory Medicine, Dept of Clinical Bacteriology
Pages 169-80
Language en
Keywords Alkane 1-Monooxygenase, genetics, Biodegradation, Environmental, Catechol 2,3-Dioxygenase, Dioxygenases, genetics, Enterobacter, genetics, isolation & purification, Hydrocarbons, metabolism, Kinetics, Multienzyme Complexes, genetics, Ochrobactrum, genetics, isolation & purification, Oxygenases, genetics, Petroleum, metabolism, Polymerase Chain Reaction, methods, RNA, Ribosomal, 16S, analysis, genetics, Soil Microbiology, Soil Pollutants, metabolism, Waste Disposal, Fluid
Subject categories Microbiology


In-situ bioremediation of petroleum waste sludge in landfarming sites of Motor Oil Hellas (petroleum refinery) was studied by monitoring the changes of the petroleum composition of the waste sludge, as well as the changes in the structure of the microbial community, for a time period of 14 months. The analyses indicated an enhanced degradation of the petroleum hydrocarbons in the landfarming areas. A depletion of n-alkanes of approximately 75-100% was obtained. Marked changes of the microbial communities of the landfarms occurred concomitantly with the degradation of the petroleum hydrocarbons. The results obtained from terminal restriction fragment length polymorphism (T-RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rRNA genes demonstrated that bacteria originating from the refinery waste sludge and newly selected bacteria dominated the soil bacterial community during the period of the highest degradation activity. However, the diversity of the microbial community was decreased with increased degradation of the petroleum hydrocarbons contained in the landfarms. T-RFLP fingerprints of bacteria of the genera Enterobacter and Ochrobactrum were detected in the landfarmed soil over the entire treatment period of 14 months. In contrast, the genus Alcaligenes appeared in significant numbers only within the 10 month old landfarmed soil. Genes encoding catechol 2,3-dioxygenase (subfamily I.2.A) were detected only in DNA of the untreated refinery waste sludge. However, none of the genes known to encode the enzymes alkane hydroxylase AlkB, catechol 2,3-dioxygenase (subfamily I.2.A) and naphthalene dioxygenase nahAc could be detected in DNA of the landfarmed soils.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?