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Diversity and expression of nitrogenase genes (nifH) from ectomycorrhizas of Corsican pine (Pinus nigra).

Journal article
Authors Hironari Izumi
Ian C Anderson
Ian J Alexander
Ken Killham
Edward R.B. Moore
Published in Environmental microbiology
Volume 8
Issue 12
Pages 2224-30
ISSN 1462-2912
Publication year 2006
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 2224-30
Language en
Keywords Basidiomycota, enzymology, genetics, Burkholderia, enzymology, genetics, DNA, Bacterial, analysis, Methylococcaceae, enzymology, genetics, Molecular Sequence Data, Mycorrhizae, metabolism, Nitrogenase, genetics, Phylogeny, Pinus, microbiology, Polymerase Chain Reaction, RNA, Messenger, metabolism, Sequence Analysis, DNA
Subject categories Microbiology


The diversity of bacterial nitrogenase genes (nifH) and their mRNA transcription in ectomycorrhizas of Corsican pine (Pinus nigra) were examined. DNA and RNA were extracted from surface-sterilized and non-sterilized Corsican pine roots colonized by the ectomycorrhizal (ECM) fungi, Suillus variegatus and Tomentellopsis submollis. DNA-derived nifH polymerase chain reaction (PCR) products were obtained from all samples, but only a few reverse transcription PCRs for nifH mRNA were successful, suggesting that nitrogenase genes were not always transcribed. Several different nifH sequences were detected and the bacteria actively transcribing nifH were different from those whose genes were detected through DNA-based PCR. Putative nitrogenase amino acid sequences revealed that more than half of the nifH products were derived from methylotrophic bacteria, such as Methylocella spp. The next most frequent sequence types were similar to those from Burkholderia.

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