To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Expression of chemokine (… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Expression of chemokine (C-C motif) ligand 18 in human macrophages and atherosclerotic plaques

Journal article
Authors Daniel Hägg
Fredrik J. Olson
Josefin Kjelldahl
Margareta Jernås
Dag Thelle
Lena M S Carlsson
Björn Fagerberg
Per-Arne Svensson
Published in Atherosclerosis
Volume 204
Issue 2
Pages e15-20
ISSN 1879-1484
Publication year 2009
Published at Institute of Medicine, Department of Molecular and Clinical Medicine
Pages e15-20
Language en
Links dx.doi.org/10.1016/j.atherosclerosi...
Keywords Aged, Biological Markers/blood, Carotid Artery Diseases/*genetics/immunology/surgery, Case-Control Studies, Chemokines, CC/blood/*genetics, Coronary Disease/genetics/immunology, Endarterectomy, Carotid, Female, Gene Expression Profiling/methods, Gene Frequency, Humans, Immunohistochemistry, Macrophages/*immunology, Male, Middle Aged, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Promoter Regions, Genetic
Subject categories Molecular medicine (genetics and pathology)

Abstract

OBJECTIVE: Using gene expression profiling, we aimed to identify genes that are predominantly expressed in human carotid atherosclerotic plaques. Such genes may be important in atherogenesis and pathophysiology of the plaque, and genes that encode for secreted proteins may be potential biomarkers for atherosclerosis and cardiovascular disease. METHODS: DNA microarray generated expression profiles of human carotid atherosclerotic plaques were compared to expression profiles of 80 different human tissues and cell types, to identify plaque-specific genes. RESULTS: We identified the chemokine (C-C motif) ligand 18 (CCL18) as predominantly expressed in human carotid plaque. Immunohistochemistry showed that CCL18 protein was localized to a subset of macrophages in carotid plaques. Monocyte-derived macrophages from subjects with atherosclerosis had threefold higher expression of CCL18 than macrophages from control subjects (p=0.012). Subjects with A/G genotype of the rs2015086 SNP in the promoter region of the CCL18 gene had threefold higher macrophage expression of CCL18 than subjects with A/A genotype (p=0.049), but we found no association of this SNP with an increased risk of coronary heart disease. We also compared serum levels of CCL18 from subjects with symptomatic carotid artery disease with control subjects. There were no differences in serum levels of CCL18 between the two groups, however CCL18 correlated with measurements of adiposity. CONCLUSION: CCL18 is predominantly expressed in human atherosclerotic plaques and may participate in the atherosclerotic plaque formation.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?