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Analysis of monosaccharide composition of mucin oligosaccharide alditols by high-performance anion-exchange chromatography.

Journal article
Authors Niclas G. Karlsson
Gunnar C. Hansson
Published in Analytical biochemistry
Volume 224
Issue 2
Pages 538-41
ISSN 0003-2697
Publication year 1995
Published at Institute of Medical Biochemistry
Pages 538-41
Language en
Links dx.doi.org/10.1006/abio.1995.1084
Keywords Acetylation, Animals, Anions, Boranes, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Hydrolysis, Intestine, Small, chemistry, Monosaccharides, analysis, Mucins, chemistry, Oligosaccharides, analysis, Rats, Sugar Alcohols, analysis, Trifluoroacetic Acid
Subject categories Chemistry, Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Abstract

A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re-N-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detector. The method was applied on a mixture of neutral oligosaccharides released from mucin glycopeptides of rat small intestine by alkaline borohydride. Sugars were detected down to the nanomole range and the results were compared with monosaccharide compositional analysis performed by gas chromatography of acetylated alditols.

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