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The leukocyte antigen CD43 is expressed in different cell lines of nonhematopoietic origin.

Journal article
Authors Julia Fernandez-Rodriguez
Christian X Andersson
Sirle Laos
Dan Baeckström
A Sikut
R Sikut
Gunnar C. Hansson
Published in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
Volume 23
Issue 4
Pages 193-201
ISSN 1010-4283
Publication year 2002
Published at Institute of Medical Biochemistry
Pages 193-201
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Antibodies, Monoclonal, metabolism, Antigens, CD, Antigens, CD43, Blotting, Western, Cell Line, Cell Separation, Electrophoresis, Polyacrylamide Gel, Exons, Flow Cytometry, Humans, Introns, Jurkat Cells, Microscopy, Confocal, Microscopy, Fluorescence, Plasmids, metabolism, Precipitin Tests, Reverse Transcriptase Polymerase Chain Reaction, Sialoglycoproteins, biosynthesis, metabolism, Tumor Cells, Cultured
Subject categories Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Abstract

CD43 is an abundant transmembrane sialoglycoprotein in leukocyte-type cell lines, but it has also been suggested to be present in colon adenomas and colon carcinomas. We have now shown that CD43 is expressed in a variety of cell lines of different origins (CaSKI, A549, 293, MTSV1-7, MCF7, HT-1080, Jurkat, K562, COLO 205, HT-29, Caco-2, DLD-1 and SW480). The level of expression of CD43 mRNA was analyzed by reverse transcriptase-polymerase chain reaction and that of the protein by immunoprecipitation and Western blot, flow cytometry and confocal microscopy using two monoclonal anti-CD43 antibodies (L10 and 4D2). As all cell lines expressed CD43, it is suggested that CD43 has a more fundamental function than previously believed and thus cannot be considered only as a specific leukocyte marker.

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