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Towards an electrowetting-based digital microfluidic platform for magnetic immunoassays

Journal article
Authors Vincent Schaller
Anke Sanz-Velasco
Alexei Kalaboukhov
Justin F. Schneiderman
Fredrik Öisjöen
Aldo Jesorka
Andrea Prieto Astalan
Anatol Krozer
Cristina Rusu
Peter Enoksson
Dag Winkler
Published in Lab on a Chip
Volume 9
Issue 23
Pages 3433-3436
ISSN 1473-0197
Publication year 2009
Published at
Pages 3433-3436
Language en
Subject categories Magnetism, Superconductivity, Fluid mechanics, Functional materials, Medical technology

Abstract

We demonstrate ElectroWetting-On-Dielectric (EWOD) transport and SQUID gradiometer detection of magnetic nanoparticles (MNPs) suspended in a 2 mu l de-ionized water droplet. This proof-of-concept methodology constitutes the first development step towards a highly sensitive magnetic immunoassay platform with SQUID readout and droplet-based sample handling. Magnetic AC-susceptibility measurements were performed on MNPs with a hydrodynamic diameter of 100 nm using a high-Tc dc Superconducting Quantum Interference Device (SQUID) gradiometer as detector. We observed that the signal amplitude per unit volume is 2.5 times higher for a 2 ml sample droplet compared to a 30 ml sample volume.

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