To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Steady-state migrating in… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Steady-state migrating intestinal dendritic cells induce potent inflammatory responses in naive CD4+ T cells.

Journal article
Authors S W F Milling
C D Jenkins
Ulf Yrlid
V Cerovic
H Edmond
V McDonald
M Nassar
G Macpherson
Published in Mucosal immunology
Volume 2
Issue 2
Pages 156-65
ISSN 1935-3456
Publication year 2009
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 156-65
Language en
Keywords Animals, CD4-Positive T-Lymphocytes, immunology, Cell Movement, physiology, Cell Proliferation, Cells, Cultured, Cytokines, secretion, Dendritic Cells, immunology, physiology, Inflammation, immunology, Interferon-gamma, immunology, Lymph, immunology, Lymph Node Excision, methods, Lymphocyte Activation, immunology, Lymphocyte Culture Test, Mixed, methods, Lymphocytes, immunology, Male, Mesentery, immunology, surgery, Rats, Th1 Cells, immunology
Subject categories Immunobiology


Steady-state dendritic cells (DCs) migrating in the lymph from the intestine induce tolerance to harmless intestinal antigens, preventing inflammatory responses. To determine if such DCs are inherently tolerogenic we collected intestinal lymph DCs (L-DCs) by cannulation of the thoracic duct of rats after mesenteric lymphadenectomy, and examined their capacity to activate naive CD4+ lymphocytes in an allogeneic mixed leucocyte reaction. L-DCs stimulated strong proliferative responses, induced secretion of inflammatory cytokines including interferon-gamma, and induced FoxP3-positive lymphocytes to divide. To determine if the activated CD4+ T cells had been tolerized, they were rested and restimulated with irradiated splenocytes. The restimulated CD4+ T cells again proliferated and secreted inflammatory cytokines. These data demonstrate that the DCs, which migrate from the intestine in the steady state, are paradoxically able to induce strong inflammatory responses from naive T cells, despite their role in the maintenance of oral tolerance.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?