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Multiplex real-time PCR for detection of respiratory tract infections

Journal article
Authors Johan Westin
Robin Brittain-Long
Sanna Nord
Sigvard Olofsson
Lars-Magnus Andersson
Magnus Lindh
Published in Journal of Clinical Virology
Volume 41
Issue 1
Pages 53-56
ISSN 1386-6532
Publication year 2008
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 53-56
Language en
Links dx.doi.org/10.1016/j.jcv.2007.10.02...
Keywords Molecular diagnostics, Respiratory infection, Real-time PCR, Taqman
Subject categories Microbiology in the medical area

Abstract

Background Broad diagnostics of respiratoryinfection by molecular assays has not yet won acceptance due to technical difficulties and high costs. Objectives To evaluate clinical applicability of multiplexreal-timePCR. Study design An assay targeting influenza virus A (IfA) and B (IfB), parainfluenza 1-3 (PIV), human metapneumovirus (MPV), respiratory syncytial virus (RSV), rhinovirus (RV), enterovirus (EV), adenovirus (AdV), human coronaviruses (229E, OC43, NL63), M. pneumoniae and Ch. pneumoniae was developed and run daily on consecutive clinical nasopharyngeal swab samples. Results An etiology was identified in 48% of the 954 samples, with IfA in 25%, RV in 20%, MPV in 10% and M. pneumoniae in 10% of the positive. By a rational procedure costs could be reduced and the customer price set relatively low (€33 per sample). Conclusion Streamlined testing and cost limitation is achievable and probably critical for implementation of a broad molecular diagnostics of respiratoryinfections.

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