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Galectin-3 functions as an opsonin and enhances the macrophage clearance of apoptotic neutrophils.

Journal article
Authors Anna Karlsson
Karin Christenson
Mustafa Matlak
Åse Björstad
Kelly Brown
Esbjörn Telemo
Emma Salomonsson
Hakon Leffler
Johan Bylund
Published in Glycobiology
Volume 19
Issue 1
Pages 16-20
ISSN 1460-2423
Publication year 2009
Published at Institute of Medicine, Department of Rheumatology and Inflammation Research
Pages 16-20
Language en
Links dx.doi.org/10.1093/glycob/cwn104
Keywords Apoptosis, immunology, Binding Sites, Cells, Cultured, Galectin 3, metabolism, Humans, Macrophage Activation, Macrophages, metabolism, Neutrophils, metabolism, Opsonin Proteins, metabolism
Subject categories Medical and Health Sciences

Abstract

Galectin-3, a beta-galactoside binding, endogenous lectin, takes part in various inflammatory events and is produced in substantial amounts at inflammatory foci. We investigated whether extracellular galectin-3 could participate in the phagocytic clearance of apoptotic neutrophils by macrophages, a process of crucial importance for termination of acute inflammation. Using human leukocytes, we show that exogenously added galectin-3 increased the uptake of apoptotic neutrophils by monocyte-derived macrophages (MDM). Both the proportion of MDM that engulfed apoptotic prey and the number of apoptotic neutrophils that each MDM engulfed were enhanced in the presence of galectin-3. The effect was lactose-inhibitable and required galectin-3 affinity for N-acetyllactosamine, a saccharide typically found on cell surface glycoproteins, since a mutant lacking this activity was without effect. The enhanced uptake relied on the presence of galectin-3 during the cellular interaction and was paralleled by lectin binding to apoptotic cells as well as MDM in a lactose-dependent manner. These findings suggest that galectin-3 functions as a bridging molecule between phagocyte and apoptotic prey, acting as an opsonin. The process of clearance, whereby apoptotic neutrophils are removed by macrophages, is crucial for the resolution of acute inflammation and our data imply that the increased levels of galectin-3 often found at inflammatory sites could potently affect this process.

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