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Imaging TOF-SIMS of rat kidney prepared by high-pressure freezing.

Journal article
Authors Håkan Nygren
Katrin Börner
Per Malmberg
Elke Tallarek
Birgit Hagenhoff
Published in Microscopy research and technique
Volume 68
Issue 6
Pages 329-34
ISSN 1059-910X
Publication year 2005
Published at Institute of Anatomy and Cell Biology
Pages 329-34
Language en
Keywords Animals, Atmospheric Pressure, Cytological Techniques, instrumentation, methods, Diagnostic Imaging, instrumentation, methods, Freezing, Image Processing, Computer-Assisted, Kidney, cytology, metabolism, Rats, Spectrometry, Mass, Secondary Ion, methods
Subject categories Medical and Health Sciences


Phosphocholine, potassium ions, and sodium ions were localized in rat kidney with imaging TOF-SIMS. Tissue preparation was performed with high-pressure freezing, freeze-fracturing and freeze-drying. The distribution of sodium ions was visualized by imaging the signal at m/z 23 of positively charged secondary ions, and the distribution of potassium ions was visualized by imaging the signal at m/z 39. Potassium was found localized within cells of the proximal tubulus epithelium and within cells of the glomeruli. High signals of sodium ions were seen in the interstitial tissue and also in epithelial cells of the collecting ducts and in glomeruli. The overlay image showed that the distribution of sodium ions and potassium ions were largely complementary with color mixing in glomeruli and in the interstitium surrounding proximal tubules. The ion distribution was further analyzed by correlation analysis. Phosphocholine-containing phospholipids were visualized by imaging the phosphocholine head group at m/z 184 of positively charged ions. The m/z 184 signal shows a ubiquitous distribution with a high intensity of phosphocholine in epithelial cells. Overlay image of m/z 184, m/z 39, and m/z 23 and multivariate analysis showed that the localization of high levels of phosphocholine colocalizes with high levels of potassium ions, as expected for an ion with intracellular localization.

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