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High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry.

Journal article
Authors Marcus Ståhlman
CS Ejsing
K Tarasov
Jeanna Perman
Jan Borén
K Ekroos
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume 877
Issue 26
Pages 2664–2672
ISSN 1570-0232
Publication year 2009
Published at Wallenberg Laboratory
Institute of Medicine, Department of Molecular and Clinical Medicine
Pages 2664–2672
Language en
Links dx.doi.org/10.1016/j.jchromb.2009.0...
Subject categories Medical and Health Sciences

Abstract

Technological advances in mass spectrometry and meticulous method development have produced several shotgun lipidomic approaches capable of characterizing lipid species by direct analysis of total lipid extracts. Shotgun lipidomics by hybrid quadrupole time-of-flight mass spectrometry allows the absolute quantification of hundreds of molecular glycerophospholipid species, glycerolipid species, sphingolipid species and sterol lipids. Future applications in clinical cohort studies demand detailed lipid molecule information and the application of high-throughput lipidomics platforms. In this review we describe a novel high-throughput shotgun lipidomic platform based on 96-well robot-assisted lipid extraction, automated sample infusion by mircofluidic-based nanoelectrospray ionization, and quantitative multiple precursor ion scanning analysis on a quadrupole time-of-flight mass spectrometer. Using this platform to compile comprehensive lipid arrays associated with metabolic dysfunctions is a powerful strategy for pinpointing the mechanistic details by which alterations in tissue-specific lipid metabolism are directly linked to the etiology of many lipid-mediated disorders.

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