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alpha 2,3/alpha 2,6-sialylation of N-glycans: non-synonymous signals with marked developmental regulation in bovine reproductive tracts

Journal article
Authors Hans-Christian Siebert
Jimmy Rosen
Kamil Seyrek
Herbert Kaltner
Sabine André
Nicolai V. Bovin
Per-Georg Nyholm
Fred Sinowatz
Hans-Joachim Gabius
Published in Biochimie
Volume 88
Issue 5
Pages 399-410
ISSN 0300-9084
Publication year 2006
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 399-410
Language en
Links dx.doi.org/10.1016/j.biochi.2005.09...
Keywords beta-galactoside alpha-2,6-sialyltransferase, carbohydrate-binding-specificity, sambucus-nigra agglutinin, repeat-type galectin-4, mechanics force-field, sialic-acid residues, colon-cancer cells, molecular-mechanics, maackia-amurensis, in-vitro, conformation sugar molecular mechanics
Subject categories Cell and molecular biology, Chemistry

Abstract

The glycan part endows cellular glycoconjugates with significant potential for biological recognition. N-Glycan branches often end with α2,3/α2,6-sialylation, posing the question whether and how placement of the sialic acid at 3´- or 6´-acceptor positions of galactose has cell biological relevance. As attractive model to study developmental regulation we monitored the expression of α2,3/α2,6-sialylated determinants in fetal and adult bovine testes and ovaries by lectin histochemistry. Distinct expression patterns were detected in both organ types. Oocyte staining, as a prominent example, was restricted to the presence of α2,6-sialylated glycans. Treatment with sialidase abolished binding and thus excluded sulfate esters as lectin targets. We added computer simulations to rationalize the observed evidence for non-random expression of the two closely related sialylgalactose isomers. Extensive molecular mechanics and molecular dynamics calculations reveal that the seemingly minor shift of the glycosidic bond from the α2,3 position to the α2,6 configuration causes significant shape and flexibility changes. They give each disaccharide its own characteristic meaning as signal in the sugar code.

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