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Identification of Functional Prolactin (PRL) Receptor Gene Expression: PRL Inhibits Lipoprotein Lipase Activity in Human White Adipose Tissue

Journal article
Authors Charlotte Ling
Louise Svensson
Birgitta Odén
Birgitta Weijdegård
Barbro Edén
Staffan Edén
Håkan Billig
Published in The Journal of Clinical Endocrinology & Metabolism
Volume 88
Issue 4
Pages 1804–1808
ISSN 0013-7227
Publication year 2003
Published at Wallenberg Laboratory
Institute for the Health of Women and Children, Dept of Obstetrics and Gynaecology
Institute of Physiology and Pharmacology, Dept of Physiology
Pages 1804–1808
Language en
Keywords Prolactin receptor, lipoprotein lipase, human adipose tissue
Subject categories Physiology


During lactation serum levels of prolactin (PRL) are elevated, and the activity of lipoprotein lipase (LPL) is decreased in the adipose tissue and increased in the mammary gland. However, PRL has been suggested to affect the adipose tissue in an indirect fashion during lactation. In the present study, we demonstrated expression of four PRL receptor (PRLR)mRNA isoforms (L, I, S1a, and S1b) in human sc abdominal adipose tissue and breast adipose tissue using RT-PCR/Southern blot analysis. In addition, L-PRLR [relative molecular mass (Mr) 90,000] and I-PRLR (Mr 50,000) protein expression was detected in human sc abdominal adipose tissue and breast adipose tissue using immunoblot analysis. Two additional protein bands with the molecular weight Mr 40–35,000 were also detected. The direct effect of PRL on the regulation of LPL activity in human abdominal adipose tissue cultured in vitro was investigated. PRL (500 ng/ml) reduced the LPL activity in human adipose tissue to 31 +/- 7.7%, compared with control.GH (100 ng/ml) also reduced the LPL activity, to 45 +/- 8.6%, compared with control. In agreement with previous studies, cortisol increased the LPL activity and GH inhibited cortisolinduced LPL activity. Furthermore, we found that PRL also inhibited the cortisol-induced LPL activity. Taken together, these results demonstrate a direct effect of PRL, via functional PRLRs, in reducing the LPL activity in human adipose tissue, and these results suggest that LPL might also be regulated in this fashion during lactation.

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