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Tubular mitochondrial alterations in neonatal rats subjected to RAS inhibition.

Journal article
Authors Daina Lasaitiene
Yun Chen
Vida Mildaziene
Zita Nauciene
Birgitta Sundelin
Bengt R Johansson
Masato Yano
Peter Friberg
Published in American journal of physiology. Renal physiology
Volume 290
Issue 5
Pages F1260-9
ISSN 0363-6127
Publication year 2006
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Institute of Medicine, Department of Molecular and Clinical Medicine
Pages F1260-9
Language en
Links dx.doi.org/10.1152/ajprenal.00150.2...
Keywords Angiotensin II, physiology, Angiotensin-Converting Enzyme Inhibitors, adverse effects, pharmacology, Animals, Animals, Newborn, Blotting, Western, Enalapril, adverse effects, pharmacology, Immunohistochemistry, Kidney Tubules, drug effects, growth & development, ultrastructure, Microscopy, Electron, Mitochondria, physiology, Na(+)-K(+)-Exchanging ATPase, biosynthesis, Rats, Rats, Wistar
Subject categories Medical and Health Sciences

Abstract

Pharmacological interruption of the angiotensin II (ANG II) type 1 receptor signaling during nephrogenesis in rats perturbs renal tubular development. This study aimed to further investigate tubular developmental defects in neonatal rats subjected to ANG II inhibition with enalapril. We evaluated tubular ultrastructural changes using electron microscopy and estimated spectrophotometrically activity or concentrations of succinate dehydrogenase (SDH), cytochromes a and c, which are components of mitochondrial respiratory chain, on postnatal days 2 and 9 (PD2 and PD9). Renal expression of sodium-potassium adenosinetriphosphatase (Na(+)-K(+)-ATPase) and two reflectors of mitochondrial biogenesis [mitochondrial transcription factor A (TFAM) and translocase of outer mitochondrial membrane 20 (TOM20)] also were studied using Western immunoblotting and immunohistochemistry. Enalapril disrupted inner mitochondrial membranes of developing cortical and medullary tubular cells on PD2 and PD9. These findings were paralleled by impaired mitochondrial respiratory function, as revealed from the changes in components of the mitochondrial respiratory chain, such as decreased cytochrome c level in the cortex and medulla on PD2 and PD9, decreased cytochrome a level in the cortex and medulla on PD2, and diminished cortical SDH activity on PD2 and PD9. Moreover, tubular expression of the most active energy-consuming pump Na(+)-K(+)-ATPase was decreased by enalapril treatment. Renal expression of TFAM and TOM20 was not altered by neonatal enalapril treatment. Because nephrogenesis is a highly energy-demanding biological process, with the energy being utilized for renal growth and transport activities, the structural-functional alterations of the mitochondria induced by neonatal enalapril treatment may provide the propensity for the tubular developmental defect.

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