To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

ASK1 inhibits astroglial … - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

ASK1 inhibits astroglial development via p38 mitogen-activated protein kinase and promotes neuronal differentiation in adult hippocampus-derived progenitor cells.

Journal article
Authors Roland Faigle
Anke Brederlau
Muna Elmi
Yvonne Arvidsson
Tatsuo S Hamazaki
Hidetaka Uramoto
Keiko Funa
Published in Molecular and cellular biology
Volume 24
Issue 1
Pages 280-93
ISSN 0270-7306
Publication year 2004
Published at Institute of Anatomy and Cell Biology
Pages 280-93
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Adenoviridae, Animals, Astrocytes, metabolism, Cell Differentiation, physiology, Genetic Vectors, Hippocampus, metabolism, MAP Kinase Kinase Kinase 5, MAP Kinase Kinase Kinases, genetics, metabolism, Mitogen-Activated Protein Kinases, metabolism, Neuroglia, metabolism, Neurons, metabolism, Promoter Regions (Genetics), Rats, Transduction, Genetic, p38 Mitogen-Activated Protein Kinases
Subject categories Medical and Health Sciences

Abstract

The mechanisms controlling differentiation and lineage specification of neural stem cells are still poorly understood, and many of the molecules involved in this process and their specific functions are yet unknown. We investigated the effect of apoptosis signal-regulating kinase 1 (ASK1) on neural stem cells by infecting adult hippocampus-derived rat progenitors with an adenovirus encoding the constitutively active form of ASK1. Following ASK1 overexpression, a significantly larger number of cells differentiated into neurons and a substantial increase in Mash1 transcription was observed. Moreover, a marked depletion of glial cells was observed, persisting even after additional treatment of ASK1-infected cultures with potent glia inducers such as leukemia inhibitory factor and bone morphogenetic protein. Analysis of the promoter for glial fibrillary acidic protein revealed that ASK1 acts as a potent inhibitor of glial-specific gene transcription. However, the signal transducers and activators of transcription 3 (STAT3)-binding site in the promoter was dispensable, while the activation of p38 mitogen-activated protein kinase was crucial for this effect, suggesting the presence of a novel mechanism for the inhibition of glial differentiation.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?