To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Neuroblastoma-specific cy… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Neuroblastoma-specific cytotoxicity mediated by the Mash1-promoter and E. coli purine nucleoside phosphorylase.

Journal article
Authors Yvonne Arvidsson
Venil Sumantran
Fujiko Watt
Hidetaka Uramoto
Keiko Funa
Published in Pediatric blood & cancer
Volume 44
Issue 1
Pages 77-84
ISSN 1545-5009
Publication year 2005
Published at Institute of Laboratory Medicine, Dept of Pathology
Institute of Anatomy and Cell Biology
Pages 77-84
Language en
Keywords Basic Helix-Loop-Helix Transcription Factors, Cell Death, DNA-Binding Proteins, genetics, pharmacology, Escherichia coli, enzymology, Gene Expression Regulation, Neoplastic, Gene Therapy, Gene Transfer Techniques, Genes, Transgenic, Suicide, Helix-Loop-Helix Motifs, Humans, Neuroblastoma, genetics, pathology, Promoter Regions (Genetics), Purine-Nucleoside Phosphorylase, pharmacology, Transcription Factors, genetics, pharmacology, Tumor Cells, Cultured
Subject categories Medical and Health Sciences


BACKGROUND: Neuroblastoma is derived from cells of neural crest origin and often expresses the transcription factor human achaete-scute homolog 1 (HASH1). The aim of this study was to selectively kill neuroblastoma cells by expressing the suicide gene E. coli purine nucleoside phosphorylase (PNP) under the control of the Mash1 promoter, the murine homolog of HASH1. PROCEDURE: The E. coli PNP gene regulated by the Mash1 promoter was cloned into an expression vector and transfected into neuroblastoma and non-neuroblastoma cell lines. After addition of the prodrug M2-fluoroadenine 9-beta-D-arabinofuranoside (F-araA) the cell-specific toxicity was examined. To optimize the cell specific activity, different sizes of the Mash1 promoter were analyzed in neuroblastoma cell lines and compared with the activity in non-neuroblastoma cells. RESULTS: Estimated as the percentages of CMV enhancer-promoter, the activity was significantly higher in the neuroblastoma cells, ranging from 17 to 58% when the shortest and the most active promoter was measured. The non-neuroblastoma cells yielded only 1-6% of the CMV promoter activity. When the shortest Mash1 promoter was combined with the E. coli PNP gene the cytotoxicity was 65% in the neuroblastoma cells with low cell death in the non-neuroblastoma cell lines, relative to the cytotoxicity where the E.coli PNP gene was regulated by the strong but non-specific CMV enhancer-promoter. CONCLUSIONS: We show here that the Mash1 promoter regulating the PNP gene confers a cell-type selective toxicity in neuroblastoma cell lines. These results indicate the feasibility to use the Mash1 promoter for regulating E.coli PNP expression in gene-directed enzyme prodrug therapy (GDEPT) of neuroblastoma.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?