To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

A novel MASH1 enhancer wi… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

A novel MASH1 enhancer with N-myc and CREB-binding sites is active in neuroblastoma.

Journal article
Authors Fujiko Watt
Rie Watanabe
Weiwen Yang
N Ågren
Yvonne Arvidsson
Keiko Funa
Published in Cancer gene therapy
Volume 14
Issue 3
Pages 287-96
ISSN 0929-1903
Publication year 2007
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 287-96
Language en
Keywords Animals, Basic Helix-Loop-Helix Transcription Factors, genetics, metabolism, Binding Sites, Chromatin Immunoprecipitation, Cyclic AMP Response Element-Binding Protein, metabolism, Electrophoretic Mobility Shift Assay, Enhancer Elements (Genetics), Gene Expression Regulation, Neoplastic, Humans, Mice, Neuroblastoma, genetics, metabolism, pathology, Promoter Regions (Genetics), Proto-Oncogene Proteins c-myc, genetics, metabolism, Regulatory Sequences, Nucleic Acid, Tumor Cells, Cultured
Subject categories Medical and Health Sciences


Neuroblastoma is one of the most common solid tumors in childhood. With the aim of developing a targeting vector for neuroblastoma, we cloned and characterized an enhancer in the 5'-flanking regions of the MASH1 gene by a random-trap method from a 36 kb cosmid DNA. The enhancer-containing clone was identified by the expression of GFP when transfected into neuroblastoma cell lines. The enhancer-luciferase activity is higher in neuroblastoma cell lines, IMR32, BE2 and SH-SY5Y, compared with those in non-neuroblastoma cell lines, U1242 glioma, N417 small cell lung cancer and EOMA hemangioma. The core enhancer was determined within a 0.2 kb fragment, yielding three- to fourfold higher activity than that of the MASH1 promoter alone in IMR32 and BE2. This area possesses GATA- and CREB-binding sites, as well as the E-box. EMSA on this area demonstrated that CREB/ATF could bind the DNA. Chromatin immunoprecipitation assay revealed that N-myc, CREB, and co-activators CBP and PCAF, but not HDAC1, are bound to the core enhancer at the same time as the co-activators and N-myc bind to the promoter. This supports the idea that the commonly overexpressed genes HASH1 and N-myc are regulated in concert, confirming their importance as prognostic markers or targets for therapy.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?