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Peritoneal dialysis fluid-induced angiogenesis in rat mesentery is increased by lactate in the presence or absence of glucose.

Journal article
Authors Ann Albrektsson
Farhan Bazargani
Anders Wieslander
Magnus Braide
Published in ASAIO journal (American Society for Artificial Internal Organs : 1992)
Volume 52
Issue 3
Pages 276-81
ISSN 1058-2916
Publication year 2006
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 276-81
Language en
Keywords Animals, Bicarbonates, pharmacology, Buffers, Dialysis Solutions, pharmacology, Glucose, pharmacology, Hydrogen-Ion Concentration, Immunohistochemistry, Lactates, pharmacology, Macrophages, Peritoneal, drug effects, Male, Mesentery, blood supply, Microscopy, Fluorescence, Neovascularization, Physiologic, drug effects, Peritoneal Dialysis, methods, Pyruvic Acid, pharmacology, Rats, Rats, Sprague-Dawley, Time Factors
Subject categories Medical and Health Sciences


Angiogenesis may be an important mechanism behind the functional deterioration of the peritoneum leading to ultrafiltration failure in peritoneal dialysis. The present study was designed to compare the angiogenic properties of lactate-, bicarbonate-, and pyruvate-buffered fluids, evaluated separately with and without glucose. Five different fluids (lactate and bicarbonate with and without 2.5% glucose and pyruvate without glucose) were studied for 5 weeks of twice-daily injections in rats. The respective buffers (40 mmol/l) were adjusted to pH 7.2, and sodium, chloride, calcium, and magnesium were present at standard concentrations. The mesenteric window model, based on observation of the translucent peritoneal sections of the small intestine mesentery, was used for immunohistochemical imaging of microvessels (RECA-1 antigen) and macrophages (ED1 and ED2 antigens). All fluids induced angiogenesis as compared with untreated controls. The lactate-buffered fluids induced larger vascularized zones than did their bicarbonate- and pyruvate-buffered counterparts. Angiogenesis was accompanied by a local recruitment of ED1 macrophages from blood. Addition of glucose to the lactate- and bicarbonate-buffered fluids did not seem to alter their pro-angiogenic properties. In conclusion, intraperitoneal exposure to lactate buffer, compared with bicarbonate, stimulates angiogenesis in the presence or absence of glucose.

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