To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Computerized determinatio… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Computerized determination of adipocyte size.

Journal article
Authors Tom Björnheden
Bozena Jakubowicz
Max Levin
Birgitta Odén
Staffan Edén
Lars Sjöström
Malin Lönn
Published in Obesity research
Volume 12
Issue 1
Pages 95-105
ISSN 1071-7323
Publication year 2004
Published at Wallenberg Laboratory
Institute of Internal Medicine, Dept of Body Composition and Metabolism
Institute of Physiology and Pharmacology, Dept of Physiology
Pages 95-105
Language en
Links www.ncbi.nlm.nih.gov/pubmed/1474284...
Keywords Adipocytes, cytology, Adult, Biopsy, statistics & numerical data, Cell Separation, Cell Size, Computers, Female, Humans, Male, Microscopy, methods, Middle Aged, Software, Video Recording
Subject categories Physiology, Endocrinology

Abstract

OBJECTIVE: Fat cell size is a fundamental parameter in the study of adipose tissue metabolism, because it markedly influences the cellular rates of metabolism. Previous techniques for the sizing of adipocytes are often complicated or time-consuming. The aim of this study was to develop a new, computerized method for rapid and accurate determination of adipocyte size in a cell suspension obtained by incubating human or rat adipose tissue biopsies with collagenase. RESEARCH METHODS AND PROCEDURES: The cell suspension was placed between a siliconized glass slide and a cover slip. Using the reference method [designated as (R)], the cell diameters were determined manually using a microscope with a calibrated ocular. The new method presented here [designated as (C)] was based on computerized image analysis. RESULTS: After two well-defined corrective adjustments, measurements with (R) and (C) agreed very well. The small remaining differences seemed, in fact, to depend on inconsistencies in (R). DISCUSSION: We propose that (C) constitutes a valuable tool to study fat cell size, because this method is fast and allows the assessment of a sufficient number of cells to get reliable data on size distribution. Furthermore, images of cell preparations may be stored for future reference.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?