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Irradiation at 634 nm releases nitric oxide from human monocytes

Journal article
Authors Ann Lindgård
Lillemor Mattsson Hultén
L. Svensson
Bassam Soussi
Published in Lasers Med Sci
Volume 22
Issue 1
Pages 30-6
ISSN 0268-8921 (Print)
Publication year 2007
Published at Wallenberg Laboratory
Institute of Clinical Sciences
Pages 30-6
Language en
Keywords Adenosine Triphosphate/biosynthesis, Humans, *Laser Therapy, Low-Level, Monocytes/*metabolism/*radiation effects, Nitric Oxide/*metabolism, Reactive Oxygen Species/metabolism
Subject categories Medical and Health Sciences


Previous studies have shown that irradiation at 634 nm decreases the release of extracellular reactive oxygen species (ROS) without affecting viability in human monocytes. Here, we examined the effect of irradiation at 634 nm on the release of nitric oxide (NO), activation of inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS), and release of intracellular ROS. Chemiluminescence assays were used to measure NO release, intracellular ROS, and adenosine triphosphate levels (to assess cell viability). Levels of iNOS and eNOS mRNA were analyzed using PCR. Irradiation resulted in elevated levels of NO but had no effect on iNOS or eNOS. Irradiation also caused a decrease in levels of intracellular ROS and had no effect on cell viability. Our studies indicate that irradiation at 634 nm releases NO, possibly from a preformed store, and reduces the production of intracellular ROS without affecting cell viability. Irradiation at 634 nm may have a wide range of clinical applications, including a reduction in oxidative stress-mediated injury in the vasculature.

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