To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Regulation and splicing o… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Regulation and splicing of scavenger receptor class B type I in human macrophages and atherosclerotic plaques

Journal article
Authors Per-Arne Svensson
Mikael C. O. Englund
Magnus S C Snäckestrand
Daniel Hägg
Bertil Ohlsson
V. Stemme
Lillemor Mattsson Hultén
Dag Thelle
Björn Fagerberg
Olov Wiklund
Lena M S Carlsson
Björn Carlsson
Published in BMC Cardiovasc Disord
Volume 5
Pages 25
ISSN 1471-2261 (Electronic)
Publication year 2005
Published at Wallenberg Laboratory
Institute of Internal Medicine, Dept of Medicine
Institute of Internal Medicine, Dept of Body Composition and Metabolism
Pages 25
Language en
Links dx.doi.org/10.1186/1471-2261-5-25
Keywords Adult, Alternative Splicing, Amino Acid Motifs, Amino Acid Sequence, Atherosclerosis/etiology/metabolism, Base Sequence, Cell Hypoxia, Cells, Cultured, Female, Gene Expression Regulation/drug effects, Humans, Lipoproteins, LDL/pharmacology, Lysosome-Associated Membrane Glycoproteins/chemistry/genetics/metabolism, Macrophages/drug effects/*metabolism, Male, Molecular Sequence Data, Protein Isoforms, RNA, Messenger/metabolism, Scavenger Receptors, Class B/chemistry/genetics/*metabolism, Sialoglycoproteins/chemistry/genetics/metabolism, src Homology Domains
Subject categories Basic Medicine, Medical and Health Sciences

Abstract

BACKGROUND: The protective role of high-density lipoprotein (HDL) in the cardiovascular system is related to its role in the reverse transport of cholesterol from the arterial wall to the liver for subsequent excretion via the bile. Scavenger receptor class B type I (SR-BI) binds HDL and mediates selective uptake of cholesterol ester and cellular efflux of cholesterol to HDL. The role of SR-BI in atherosclerosis has been well established in murine models but it remains unclear whether SR-BI plays an equally important role in atherosclerosis in humans. The aim of this study was to investigate the expression of SR-BI and its isoforms in human macrophages and atherosclerotic plaques. METHODS: The effect of hypoxia and minimally modified low-density lipoprotein (mmLDL), two proatherogenic stimuli, on SR-BI expression was studied in human monocyte-derived macrophages from healthy subjects using real-time PCR. In addition, SR-BI expression was determined in macrophages obtained from subjects with atherosclerosis (n = 15) and healthy controls (n = 15). Expression of SR-BI isoforms was characterized in human atherosclerotic plaques and macrophages using RT-PCR and DNA sequencing. RESULTS: SR-BI expression was decreased in macrophages after hypoxia (p < 0.005). In contrast, SR-BI expression was increased by exposure to mmLDL (p < 0.05). There was no difference in SR-BI expression in macrophages from patients with atherosclerosis compared to controls. In both groups, SR-BI expression was increased by exposure to mmLDL (p < 0.05). Transcripts corresponding to SR-BI and SR-BII were detected in macrophages. In addition, a third isoform, referred to as SR-BIII, was discovered. All three isoforms were also expressed in human atherosclerotic plaque. Compared to the other isoforms, the novel SR-BIII isoform was predicted to have a unique intracellular C-terminal domain containing 53 amino acids. CONCLUSION: We conclude that SR-BI is regulated by proatherogenic stimuli in humans. However, we found no differences between subjects with atherosclerosis and healthy controls. This indicates that altered SR-BI expression is not a common cause of atherosclerosis. In addition, we identified SR-BIII as a novel isoform expressed in human macrophages and in human atherosclerotic plaques.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?