To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Regulatory T cells specif… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Regulatory T cells specifically suppress conventional CD8 alpha beta T cells in intestinal tumors of APC(Min/+) mice

Journal article
Authors Louis Szeponik
Paulina Akeus
William Rodin
Sukanya Raghavan
Marianne Quiding-Järbrink
Published in Cancer Immunology, Immunotherapy
Volume 69
Pages 1279-1292
ISSN 0340-7004
Publication year 2020
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 1279-1292
Language en
Keywords Regulatory T cells, APCmin, +, Colon cancer, Tumor-infiltrating, lymphocytes, Anti-tumor immunity, chemokine production, colon-cancer, accumulation, promote, gamma, angiogenesis, mechanisms, landscape, depletion, responses, Oncology, Immunology
Subject categories Microbiology in the medical area


The presence of activated T cells in colorectal cancer tissues is a strong predictor of patient survival. Our previous studies have shown that regulatory T cells (Treg) are able to reduce T cell transendothelial migration in vitro and accumulation of effector T cells in intestinal tumors in vivo in the murine APC(Min/+) model for microsatellite stable intestinal tumors. In this study, we investigated the effect of Treg depletion on the density and effector functions of different TCR alpha beta(+) and TCR gamma delta(+) T cell populations in intestinal tumors. We used the APC(Min/+)\DEREG mouse model, which harbor a diphtheria toxin receptor under the control of the FOXP3 promoter, to deplete Treg in tumor bearing mice. We found that the density of conventional TCR alpha beta(+)CD8 alpha beta(+) T cells was significantly increased in Treg-depleted tumors in comparison with Treg-proficient tumors. Furthermore, TCR alpha beta(+)CD8 alpha beta(+) T cells showed increased proliferation and activation as well as increased Granzyme B and IFN-gamma production in Treg-depleted tumors. In sharp contrast, the densities and effector functions of TCR alpha beta(+)CD8 alpha alpha(+) T cells and TCR gamma delta(+) T cells remained unchanged by Treg depletion. We also documented a distinct population of IL-17A(+)TNF(+) TCR gamma delta(+)CD8(-) T cells in tumors, which were not affected by Treg depletion. We conclude that Treg depletion affects only conventional TCR alpha beta(+)CD8 alpha beta(+) T cells in intestinal tumors, while unconventional T cells and T cells in unaffected tissue are not altered. Immunotherapies aimed at depleting Treg from tumors may thus be a viable option for reinvigoration of conventional cytotoxic T cells with a Th1 cytokine profile.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?