To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Homogeneous Differential … - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Homogeneous Differential Magnetic Assay

Journal article
Authors S. Sepehri
T. Z. G. de la Torre
Justin F. Schneiderman
J. Blomgren
A. Jesorka
C. Johansson
M. Nilsson
J. Albert
M. Stromme
D. Winkler
A. Kalaboukhovf
Published in Acs Sensors
Volume 4
Issue 9
Pages 2381-2388
ISSN 2379-3694
Publication year 2019
Published at Institute of Neuroscience and Physiology
Pages 2381-2388
Language en
Links dx.doi.org/10.1021/acssensors.9b009...
Keywords homogeneous differential magnetic assay, magnetic nanoparticle, volumetric detection, rolling circle, amplified DNA-molecules, sensitive detection, nanoparticles, diagnostics, amplification, bioassay, devices, markers, spores, Chemistry, Science & Technology - Other Topics, rmany, v380, p205
Subject categories Chemical Sciences

Abstract

Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?