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Free lipid and computerized determination of adipocyte size.

Journal article
Authors Henrik Svensson
Daniel Olausson
Agneta Holmäng
Eva Jennische
Staffan Edén
Malin Lönn
Published in Adipocyte
Volume 7
Issue 3
Pages 180-182
ISSN 2162-397X
Publication year 2018
Published at Institute of Neuroscience and Physiology, Department of Physiology
Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 180-182
Language en
Links dx.doi.org/10.1080/21623945.2018.14...
www.ncbi.nlm.nih.gov/entrez/query.f...
Subject categories Morphology, Cell and Molecular Biology

Abstract

The size distribution of adipocytes in a suspension, after collagenase digestion of adipose tissue, can be determined by computerized image analysis. Free lipid, forming droplets, in such suspensions implicates a bias since droplets present in the images may be identified as adipocytes. This problem is not always adjusted for and some reports state that distinguishing droplets and cells is a considerable problem. In addition, if the droplets originate mainly from rupture of large adipocytes, as often described, this will also bias size analysis. We here confirm that our ordinary manual means of distinguishing droplets and adipocytes in the images ensure correct and rapid identification before exclusion of the droplets. Further, in our suspensions, prepared with focus on gentle handling of tissue and cells, we find no association between the amount of free lipid and mean adipocyte size or proportion of large adipocytes.

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