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A novel ulvan lyase family with broad-spectrum activity from the ulvan utilisation loci of Formosa agariphila KMM 3901

Journal article
Authors V. R. Konasani
Chunsheng Jin
Niclas G. Karlsson
E. Albers
Published in Scientific Reports
Volume 8
ISSN 2045-2322
Publication year 2018
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Language en
Links dx.doi.org/10.1038/s41598-018-32922...
Keywords persicivirga-ulvanivorans, sulfated polysaccharides, signal peptides, green seaweeds, uronic-acid, web server, algae, degradation, extracts, oligosaccharides, Science & Technology - Other Topics
Subject categories Biological Sciences

Abstract

Ulvan, which is one of the major structural polysaccharides of the cell walls of green macroalgae, is degraded by ulvan lyases via the beta-elimination mechanism with the release of oligosaccharides that have unsaturated 4-deoxy-L-threo-hex-4-enopyranosiduronic acid (Delta) at the non-reducing end. These ulvan lyases belong to the PL24 or PL25 or PL28 family in the CAZy database. In this study, we identify and biochemically characterise a periplasmic novel broad-spectrum ulvan lyase from Formosa agariphila KMM 3901. The lyase was overexpressed in Escherichia coli, and the purified recombinant enzyme depolymerised ulvan in an endolytic manner with a K-m of 0.77 mg/ml, and displayed optimum activity at 40 degrees C and pH 8. This lyase also degraded heparan sulphate and chondroitin sulphate. Detailed analyses of the end-products of the enzymatic degradation of ulvan using H-1- and C-13-NMR and LC-MS revealed an unsaturated disaccharide (Delta Rha3S) and a tetrasaccharide (Delta Rha3S-Xyl-Rha) as the principal end-products. In contrast to the previously described ulvan lyases, this novel lyase is mostly composed of alpha-helices that form an (alpha/alpha)(6) incomplete toroid domain and displays a remarkably broad-spectrum activity. This novel lyase is the first member of a new family of ulvan lyases.

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