To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Membrane Deformation Indu… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic

Journal article
Authors N. Parveen
Inga Rimkute
S. Block
Gustaf E Rydell
D. Midtvedt
Göran Larson
V. P. Hytönen
V. P. Zhdanov
A. Lundgren
F. Höök
Published in Journal of Physical Chemistry Letters
Volume 9
Issue 9
Pages 2278-2284
ISSN 1948-7185
Publication year 2018
Published at Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Pages 2278-2284
Language en
Links dx.doi.org/10.1021/acs.jpclett.8b00...
Keywords Cell membranes, Cytology, Deformation, Fluorescence microscopy, Gold nanoparticles, Lipid bilayers, Refractive index, Viruses, Affinity receptors, Cellular membranes, Functionalized gold nanoparticles, Glycosphingolipids, Nano-carriers, Norovirus, Quartz crystal microbalance with dissipation monitoring, Total internal reflection fluorescence microscopy, Drug delivery
Subject categories Pharmacology and Toxicology, Physical Chemistry

Abstract

Quartz crystal microbalance with dissipation monitoring and total internal reflection fluorescence microscopy have been used to investigate binding of norovirus-like particles (noroVLPs) to a supported (phospho)lipid bilayer (SLB) containing a few percent of H or B type 1 glycosphingolipid (GSL) receptors. Although neither of these GSLs spontaneously form domains, noroVLPs were observed to form micron-sized clusters containing typically up to about 30 VLP copies, especially for B type 1, which is a higher-affinity receptor. This novel finding is explained by proposing a model implying that VLP-induced membrane deformation promotes VLP clustering, a hypothesis that was further supported by observing that functionalized gold nanoparticles were able to locally induce SLB deformation. Because similar effects are likely possible also at cellular membranes, our findings are interesting beyond a pure biophysicochemical perspective as they shed new light on what may happen during receptor-mediated uptake of viruses as well as nanocarriers in drug delivery. © Copyright 2018 American Chemical Society.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?