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Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing.

Journal article
Authors Clinton D Orebaugh
Scott A Lujan
Adam B Burkholder
Anders R Clausen
Thomas A Kunkel
Published in Methods in molecular biology (Clifton, N.J.)
Volume 1672
Pages 329-345
ISSN 1940-6029
Publication year 2018
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 329-345
Language en
Links dx.doi.org/10.1007/978-1-4939-7306-...
www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Computational Biology, methods, DNA, isolation & purification, metabolism, DNA Repair, DNA Replication, DNA, Fungal, isolation & purification, Gene Library, Genomics, methods, High-Throughput Nucleotide Sequencing, Hydrolysis, Ribonuclease H, metabolism, Ribonucleotides, Yeasts
Subject categories Genetics, Functional genomics, Molecular biology

Abstract

Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequencing (HydEn-seq) to map ribonucleotides inserted into the genome of Saccharomyce cerevisiae strains deficient in ribonucleotide excision repair. We use this method to map several genomic features in wild-type and replicase variant yeast strains.

Page Manager: Webmaster|Last update: 9/11/2012
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