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Dual polarity MALDI imaging mass spectrometry on the same pixel points reveals spatial lipid localizations at high-spatial resolutions in rat small intestine

Journal article
Authors Ibrahim Kaya
Eva Jennische
Stefan Lange
Per Malmberg
Published in Analytical Methods
Volume 10
Issue 21
Pages 2428-2435
ISSN 1759-9660
Publication year 2018
Published at Institute of Biomedicine
Department of Chemistry and Molecular Biology
National Center for Imaging Mass Spectrometry
Pages 2428-2435
Language en
Links https://doi.org/10.1039/C8AY00645H
Keywords alzheimers-disease, matrix, 1,5-diaminonaphthalene, sublimation, tissues, ms, Chemistry, Food Science & Technology, Spectroscopy
Subject categories Spectroscopy, Food Engineering, Chemical Sciences

Abstract

Sensitive laser desorption/ionization obtained via a sublimation-coated 1,5-diaminonaphthalene (1,5-DAN) matrix allowed dual polarity MALDI-IMS analysis on the same pixel points across the jejunal mucosal region in rat small intestine which yielded high-spatial-resolution (10 m) ion images of several lipid species correlated with the same histological features.

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