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Helicobacter pylori SabA binding gangliosides of human stomach

Journal article
Authors John Benktander
Angela Barone
Miralda Madar Johansson
Susann Teneberg
Published in Virulence
Volume 9
Issue 1
Pages 738-751
ISSN 2150-5594
Publication year 2018
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 738-751
Language en
Links doi.org/10.1080/21505594.2018.14401...
Keywords gangliosides, human gastric glycosphingolipids, H. pylori SabA, glycosphingolipid characterization, microbial adhesion, group-related antigens, normal human-leukocytes, human gastric-mucosa, blood-group antigens, leukemia hl60 cells, sialyl-lewis-x, mass-spectrometry, human neutrophils, duodenal mucosae, adhesin saba, Immunology, Infectious Diseases, Microbiology
Subject categories Microbiology

Abstract

Adhesion of Helicobacter pylori to the gastric mucosa is a prerequisite for the pathogenesis of H. pylori related diseases. In this study, we investigated the ganglioside composition of human stomach as the target for attachment mediated by H. pylori SabA (sialic acid binding adhesin). Acid glycosphingolipids were isolated from human stomach and separated into subfractions, which were characterized by mass spectrometry and by binding of antibodies, bacteria, and Solanum tuberosum lectin. H. pylori SabA binding gangliosides were characterized as Neu5Ac alpha 3-neolactohexaosylceramide and Neu5Ac alpha 3-neolactooctaosylceramide, while the other acid human stomach glycosphingolipids characterized (sulfatide and the gangliosides GM3, GD3, GM1, Neu5Ac alpha 3-neolactotetraosylceramide, GD1a and GD1b) were not recognized by the bacteria. Defining H. pylori binding glycosphingolipids of the human gastric mucosa will be useful to specifically target this microbe-host interaction for therapeutic intervention.

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