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Conjugation factors controlling F-plasmid antibiotic resistance transmission

Magazine article
Authors Hanna Alalam
Fabrice Graf
Martin Palm
Marie Abadikhah
Martin Zackrisson
Matilda Mattsson
Chris Hadjineophytou
Linnèa Persson
Simon Stenberg
Payam Ghiaci
Per Sunnerhagen
Jonas Warringer
Anne Farewell
Published in BioRxiv
Publication year 2018
Published at Department of Chemistry and Molecular Biology
Centre for antibiotic resistance research, CARe
Language en
Links https://doi.org/10.1101/271254
Keywords antibiotic resistance, horizontal gene transfer
Subject categories Microbiology, Bacteriology

Abstract

The rapid horizontal transmission of many antibiotic resistance genes between bacterial host cells on conjugative plasmids is a major cause of the accelerating antibiotic resistance crisis. Preventing understanding and targeting conjugation, there currently are no experimental platforms for fast and cost-efficient screening of genetic effects on antibiotic resistance transmission by conjugation. We introduce a novel experimental framework to screen for conjugation based horizontal transmission of antibiotic resistance between >60,000 pairs of cell populations in parallel. Plasmid-carrying donor strains are constructed in high throughput. We then mix the resistance plasmid carrying donors with recipients in a design where only transconjugants can reproduce, measure growth in dense intervals and extract transmission times as the growth lag. As proof-of-principle, we exhaustively explored chromosomal genes controlling F plasmid donation within E. coli populations, by screening the Keio deletion collection at high replication. We recover all six known chromosomal gene mutants affecting conjugation and identify >50 novel factors, all of which diminish antibiotic resistance transmission. We verify 10 of the novel genes' effects in a liquid mating assay. The new framework holds great potential for exhaustive disclosing of candidate targets for helper drugs that delay resistance development in patients and societies and improves the longevity of current and future antibiotics.

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