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Transepithelial transfer of phenanthrene, but not of benzo[a]pyrene, is inhibited by fatty acids in the proximal intestine of rainbow trout (Oncorhynchus mykiss)

Journal article
Authors Stefan de Gelder
Henrik Sundh
Thamar N.M. Pelgrim
Josef D. Rasinger
Lotte van Daal
Gert Flik
Marc H.G. Berntssen
Peter H.M. Klaren
Published in Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology
Volume 204
Pages 97-105
ISSN 1532-0456
Publication year 2018
Published at Department of Biological and Environmental Sciences
Pages 97-105
Language en
Links dx.doi.org/10.1016/j.cbpc.2017.11.0...
Keywords Fatty acids, Intestinal uptake, Lipid metabolism, Oncorhynchus mykiss, Polycyclic aromatic hydrocarbons, Rainbow trout, Toxicology, Ussing chamber
Subject categories Animal physiology, Aquaculture

Abstract

© 2017 Elsevier Inc. The inclusion of vegetable oils in aquafeeds introduces contaminating polycyclic aromatic hydrocarbons (PAHs) in salmonids. Since lipophilic PAHs solubilize in micelles composed of lipids, bile salts and fatty acids, dietary lipid composition can alter intestinal transepithelial PAH transfer. We studied the uptake of two PAHs, viz. benzo[a]pyrene (BaP) and phenanthrene (PHE), in rainbow trout (Oncorhynchus mykiss) intestine. We also investigated the effects of two fatty acids, viz. fish oil-derived eicosapentaenoic acid (EPA, 20:5n-3) and vegetable oil-derived oleic acid (18:1n-9) on intestinal uptake. Radiolabeled PAHs were solubilized in micelles composed of tritiated EPA and oleic acid, respectively, and administrated to intestinal segments mounted in Ussing chambers. In the absence of micelles, PHE accumulation was two times higher than BaP in the mucosal and serosal layers of proximal and distal intestine. Administration of PHE in micelles composed of oleic acid resulted in a 50% lower accumulation of PHE in the mucosal layers of the proximal intestine compared to EPA-composed micelles. Accumulation of EPA and oleic acid in the proximal intestinal mucosa correlated negatively with the transepithelial transfer of these fatty acids across the proximal intestinal epithelium. Transepithelial PHE transfer across the proximal intestine was reduced by 30% in co-exposure with EPA-composed micelles compared to 80% with oleic acid micelles. BaP was not transferred across the intestine. We conclude that the lipid composition of an aquafeed is an important determinant of PAH bioavailability. Therefore, lipid composition should be an important consideration in choosing vegetable oils as alternatives for fish oil in aquafeeds.

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