To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Tissue Factor promotes br… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Tissue Factor promotes breast cancer stem cell activity in vitro

Journal article
Authors H. Shaker
H. Harrison
R. Clarke
Göran Landberg
N. J. Bundred
H. H. Versteeg
C. C. Kirwan
Published in Oncotarget
Volume 8
Issue 16
Pages 25915-25927
ISSN 1949-2553
Publication year 2017
Published at Sahlgrenska Cancer Center
Pages 25915-25927
Language en
Links dx.doi.org/10.18632/oncotarget.1392...
https://gup.ub.gu.se/file/206891
Keywords cancer stem cells, tissue factor, breast cancer, GROWTH-FACTOR RECEPTOR, FACTOR VIIA, FACTOR EXPRESSION, SIGNALING, PATHWAY, COAGULATION, TUMORIGENICITY, INTERFERENCE, ANGIOGENESIS, ACTIVATION, RESISTANCE
Subject categories Cancer and Oncology

Abstract

Cancer stem cells (CSCs) are a subpopulation of cells that can self-renew and initiate tumours. The clotting-initiating protein Tissue Factor (TF) promotes metastasis and may be overexpressed in cancer cells with increased CSC activity. We sought to determine whether TF promotes breast CSC activity in vitro using human breast cancer cell lines. TF expression was compared in anoikis-resistant (CSC-enriched) and unselected cells. In cells sorted into of TF-expressing and TF-negative (FACS), and in cells transfected to knockdown TF (siRNA) and overexpress TF (cDNA), CSC activity was compared by (i) mammosphere forming efficiency (MFE) (ii) holoclone colony formation (Hc) and (iii) ALDH1 activity. TF expression was increased in anoikis-esistant and high ALDH1-activity T47D cells compared to unselected cells. FACS sorted TF-expressing T47Ds and TF-overexpressing MCF7s had increased CSC activity compared to TF-low cells. TF siRNA cells (MDAMB231, T47D) had reduced CSC activity compared to control cells. FVIIa increased MFE and ALDH1 in a dose-dependent manner (MDAMB231, T47D). The effects of FVIIa on MFE were abrogated by TF siRNA (T47D). Breast CSCs (in vitro) demonstrate increased activity when selected for high TF expression, when induced to overexpress TF, and when stimulated (with FVIIa). Targeting the TF pathway in vivo may abrogate CSC activity.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?