To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Multicolor immunofluoresc… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Multicolor immunofluorescence reveals that p63-and/or K5-positive progenitor cells contribute to normal breast epithelium and usual ductal hyperplasia but not to low-grade intraepithelial neoplasia of the breast

Journal article
Authors W. Boecker
Göran Stenman
T. Schroeder
U. Schumacher
T. Loning
L. Stahnke
C. Lohnert
R. Siering
A. Kuper
V. Samoilova
M. Tiemann
E. Korsching
I. Buchwalow
Published in Virchows Archiv
Volume 470
Issue 5
Pages 493-504
ISSN 0945-6317
Publication year 2017
Published at Sahlgrenska Cancer Center
Pages 493-504
Language en
Keywords Normal breast epithelium, Usual ductal hyperplasia, Low-grade intraepithelial neoplasias, carcinoma in-situ, comparative genomic hybridization, receptor-positive, cells, human airway epithelium, lobular carcinoma, estrogen-receptor, stem-cells, tubular carcinoma, expression patterns, precancerous breast, Pathology
Subject categories Pathology, Cancer and Oncology


We contend that knowledge about the cellular composition of normal breast epithelium is a prerequisite for understanding proliferative breast disease. Against this background, we used multicolor immunofluorescence to study normal breast epithelium and two types of intraepithelial proliferative breast lesion for expression of the p63, basal keratin K5, glandular keratin K8/18, SMA, ER-alpha, and Ki67. We studied eight normal breast epithelium samples, 12 cases of usual ductal hyperplasia, and 33 cases of low-grade intraepithelial neoplasia (9 flat epithelial atypia, 14 low-grade ductal carcinoma in situ and 10 cases of lobular neoplasia). Usual ductal hyperplasia showed striking similarity to normal luminal breast epithelium including p63+ and/or K5+ luminal progenitor cells and the full spectrum of luminal progeny cells. In normal breast epithelium and usual ductal hyperplasia, expression of ER-alpha was associated with lack of expression of the proliferation antigen Ki67. In contrast, we found in both types of low-grade intraepithelial neoplasia robust expression of keratin K8/18 and a positive association between ER-alpha and Ki67 expression. However, these lesions were consistently negative for p63 and/or K5. Our observational study supports the view that usual ductal hyperplasia and low-grade intraepithelial neoplasia are different entities rather than part of a spectrum of the same disease. We propose a new operational model of cell differentiation that may serve to better understand correlations between normal breast epithelium and proliferative breast diseases. From our data we conclude that p63+ and/or K5+ progenitor cells contribute to maintenance of normal epithelium and usual ductal hyperplasia, but not to low-grade intraepithelial neoplasia of the breast.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?