To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

An Adaptable High-Through… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

An Adaptable High-Throughput Technology Enabling the Identification of Specific Transcription Modulators

Journal article
Authors T. Bergbrede
Emily Hoberg
N. G. Larsson
Maria Falkenberg
Claes M Gustafsson
Published in Slas Discovery
Volume 22
Issue 4
Pages 378-386
ISSN 2472-5552
Publication year 2017
Published at Institute of Biomedicine
Pages 378-386
Language en
Links doi.org/10.1177/2472555217690326
Keywords Antiviral drugs, fluorescence methods, nucleic acid chemistry, assays, labeling, binding or purification, mitochondrial rna-polymerase, acute myeloid-leukemia, hepatitis-c virus, in-vitro, therapy, cancer, dna, pcr, Biochemistry & Molecular Biology, Biotechnology & Applied Microbiology, Chemistry
Subject categories Biochemistry and Molecular Biology, Microbiology, Chemical Sciences

Abstract

Mitochondria harbor the oxidative phosphorylation (OXPHOS) system, which under aerobic conditions produces the bulk of cellular adenosine triphosphate (ATP). The mitochondrial genome encodes key components of the OXPHOS system, and it is transcribed by the mitochondrial RNA polymerase, POLRMT. The levels of mitochondrial transcription correlate with the respiratory activity of the cell. Therefore, compounds that can increase or decrease mitochondrial gene transcription may be useful for fine-tuning metabolism and could be used to treat metabolic diseases or certain forms of cancer. We here report the establishment of a novel high-throughput assay technology that has allowed us to screen a library of 430,000 diverse compounds for effects on mitochondrial transcription in vitro. Following secondary screens facilitated by the same assay principle, we identified 55 compounds that efficiently and selectively inhibit mitochondrial transcription and that are active also in cell culture. Our method is easily adaptable to other RNA or DNA polymerases and varying spectroscopic detection technologies.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?