To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Solid phase extraction an… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Solid phase extraction and metabolic profiling of exudates from living copepods

Journal article
Authors Erik Selander
J. Heuschele
Göran M. Nylund
G. Pohnert
Henrik Pavia
O. Bjaerke
L. A. Pender-Healy
Peter Tiselius
T. Kiorboe
Published in Peerj
Volume 4
ISSN 2167-8359
Publication year 2016
Published at Department of marine sciences
Department of Biological and Environmental Sciences, Kristineberg
Language en
Keywords Copepod exudate, Metabolomics, Infochemicals, Temora longicornis, Exometabolome, planktonic copepods, centropages-typicus, swimming behavior, chemical, ecology, cues, diatom, mate, phytoplankton, longicornis, ecosystems
Subject categories Marine ecology


Copepods are ubiquitous in aquatic habitats. They exude bioactive compounds that mediate mate finding or induce defensive traits in prey organisms. However, little is known about the chemical nature of the copepod exometabolome that contributes to the chemical landscape in pelagic habitats. Here we describe the development of a closed loop solid phase extraction setup that allows for extraction of exuded metabolites from live copepods. We captured exudates from male and female Temora longicornis and analyzed the content with high resolution LC-MS. Chemometric methods revealed 87 compounds that constitute a specific chemical pattern either qualitatively or quantitatively indicating copepod presence. The majority of the compounds were present in both female and male exudates, but nine compounds were mainly or exclusively present in female exudates and hence potential pheromone candidates. Copepodamide G, known to induce defensive responses in phytoplankton, was among the ten compounds of highest relative abundance in both male and female extracts. The presence of copepodamide G shows that the method can be used to capture and analyze chemical signals from living source organisms. We conclude that solid phase extraction in combination with metabolic profiling of exudates is a useful tool to develop our understanding of the chemical interplay between pelagic organisms.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?