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Amplification and enhanced expression of the c-myc oncogene in mouse SEWA tumour cells.

Journal article
Authors M Schwab
G Ramsay
K Alitalo
H E Varmus
J M Bishop
Tommy Martinsson
Göran Levan
A Levan
Published in Nature
Volume 315
Issue 6017
Pages 345-7
ISSN 0028-0836
Publication year 1985
Published at
Pages 345-7
Language en
Keywords Animals, Cell Line, Chromosome Aberrations, genetics, Chromosome Disorders, Chromosomes, ultrastructure, DNA, Neoplasm, genetics, Gene Amplification, Gene Expression Regulation, Mice, Oncogenes, Osteosarcoma, genetics, Phosphoproteins, genetics, Proto-Oncogene Proteins c-myc, RNA, Messenger, genetics, RNA, Neoplasm, genetics, Sarcoma, Experimental, genetics
Subject categories Genetics

Abstract

SEWA tumour cells are derived from an osteosarcoma induced in an A.SW mouse by infection with polyoma virus. Cytogenetic analyses have revealed three different characteristic chromosomal abnormalities diagnostic for the presence of amplified genes: 'double minutes' (DMs), homogeneously staining chromosomal regions (HSRs) and C-bandless chromosomes (CMs; for review see ref. 2). DMs may undergo fluctuation in number depending on the conditions in which the cells grow. Their number usually increases after injection of cells into a mouse and often is reduced to undetectable levels when the cells are explanted back into tissue culture; when the cells are re-introduced into the mouse, they again acquire multiple DMs. We show here that cells of SEWA lines carrying DMs, HSRs or CMs contain amplified copies of the proto-oncogene c-myc and enhanced levels of c-myc messenger RNA and c-myc protein. DMs or CMs are the sites of c-myc amplification in two different SEWA lines.

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