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Resistance to actinomycin D and to vincristine induced in a SEWA mouse tumor cell line with concomitant appearance of double minutes and a low molecular weight protein.

Journal article
Authors B Dahllöf
Tommy Martinsson
Göran Levan
Published in Experimental cell research
Volume 152
Issue 2
Pages 415-26
ISSN 0014-4827
Publication year 1984
Published at
Pages 415-26
Language en
Keywords Animals, Cell Line, Chromosome Aberrations, Dactinomycin, pharmacology, Drug Resistance, Gene Amplification, Mice, Molecular Weight, Neoplasm Proteins, biosynthesis, Neoplasms, Experimental, genetics, Vincristine, pharmacology
Subject categories Genetics

Abstract

By increasing stepwise the drug concentration of the medium, we have induced, in a cell line of the murine SEWA tumor, resistance to actinomycin D (AMD) and vincristine (VCR) 30-50 times above the normal. In both types of resistant cells, we have revealed, by two-dimensional gel electrophoresis, a protein (MW 21K , pI 5) not found in control cells. Large fractions of the resistant cells contained double minutes (DM). In AMD-resistant cells, correlation was demonstrated between number of DM and degree of resistance. Back in AMD-free medium, resistant cells lost both the DM and the 21K protein. Cross-resistance prevailed between AMD and VCR. Cells resistant to AMD and VCR showed erratic resistance to methotrexate (MTX), but no significant resistance existed in the reverse direction.

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