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Expression, sorting, and segregation of Golgi proteins during germ cell differentiation in the testis

Journal article
Authors C. E. Au
L. Hermo
E. Byrne
J. Smirle
A. Fazel
P. H. G. Simon
R. E. Kearney
P. H. Cameron
C. E. Smith
H. Vali
Julia Fernandez-Rodriguez
K. W. Ma
T. Nilsson
J. J. M. Bergeron
Published in Molecular Biology of the Cell
Volume 26
Issue 22
Pages 4015-4032
ISSN 1059-1524
Publication year 2015
Published at Core Facilities, Centre for Cellular Imaging
Pages 4015-4032
Language en
Links dx.doi.org/10.1091/mbc.E14-12-1632
Keywords MANNOSE 6-PHOSPHATE RECEPTOR, CLATHRIN-COATED VESICLES, IN-VIVO ROLE, ENDOPLASMIC-RETICULUM, PLASMA-MEMBRANE, N-GLYCANS, 3-DIMENSIONAL, ARCHITECTURE, MAMMALIAN SPERMATOGENESIS, TARGETED DISRUPTION, CYTOPLASMIC DROPLET, Cell Biology, ATES OF AMERICA, V104, P8346, NG XM, 1982, AMERICAN JOURNAL OF ANATOMY, V163, P283
Subject categories Cell biology

Abstract

The molecular basis of changes in structure, cellular location, and function of the Golgi apparatus during male germ cell differentiation is unknown. To deduce cognate Golgi proteins, we isolated germ cell Golgi fractions, and 1318 proteins were characterized, with 20 localized in situ. The most abundant protein, GL54D of unknown function, is characterized as a germ cell-specific Golgi-localized type II integral membrane glycoprotein. TM9SF3, also of unknown function, was revealed to be a universal Golgi marker for both somatic and germ cells. During acrosome formation, several Golgi proteins (GBF1, GPP34, GRASP55) localize to both the acrosome and Golgi, while GL54D, TM9SF3, and the Golgi trafficking protein TMED7/p27 are segregated from the acrosome. After acrosome formation, GL54D, TM9SF3, TMED4/p25, and TMED7/p27 continue to mark Golgi identity as it migrates away from the acrosome, while the others (GBF1, GPP34, GRASP55) remain in the acrosome and are progressively lost in later steps of differentiation. Cytoplasmic HSP70.2 and the endoplasmic reticulum luminal protein-folding enzyme PDILT are also Golgi recruited but only during acrosome formation. This resource identifies abundant Golgi proteins that are expressed differentially during mitosis, meiosis, and postacrosome Golgi migration, including the last step of differentiation.

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