To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Treg-cell depletion promo… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Treg-cell depletion promotes chemokine production and accumulation of CXCR3(+) conventional T cells in intestinal tumors.

Journal article
Authors Paulina Akeus
Veronica Langenes
Jonas Kristensen
Astrid von Mentzer
Tim Sparwasser
Sukanya Raghavan
Marianne Quiding-Järbrink
Published in European journal of immunology
Volume 45
Issue 6
Pages 1654-66
ISSN 1521-4141
Publication year 2015
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 1654-66
Language en
Links dx.doi.org/10.1002/eji.201445058
Keywords Adenomatous Polyposis Coli Protein, metabolism, Animals, Cell Adhesion Molecules, metabolism, Chemokines, biosynthesis, Chemotaxis, immunology, Colorectal Neoplasms, immunology, metabolism, Cytokines, biosynthesis, Disease Models, Animal, Female, Intestinal Mucosa, immunology, metabolism, Lymphocyte Activation, immunology, Lymphocyte Depletion, Lymphocytes, Tumor-Infiltrating, immunology, metabolism, Mice, Phenotype, Receptors, CXCR3, metabolism, Receptors, Chemokine, metabolism, T-Lymphocyte Subsets, immunology, metabolism, T-Lymphocytes, Regulatory, immunology, Th1 Cells, immunology, metabolism, Vascular Cell Adhesion Molecule-1, metabolism
Subject categories Tumour immunology

Abstract

Colorectal cancer (CRC) is one of the most prevalent tumor types worldwide and tumor-infiltrating T cells are crucial for anti-tumor immunity. We previously demonstrated that Treg cells from CRC patients inhibit transendothelial migration of conventional T cells. However, it remains unclear if local Treg cells affect lymphocyte migration into colonic tumors. By breeding APC(Min/+) mice with depletion of regulatory T cells mice, expressing the diphtheria toxin receptor under the control of the FoxP3 promoter, we were able to selectively deplete Treg cells in tumor-bearing mice, and investigate the impact of these cells on the infiltration of conventional T cells into intestinal tumors. Short-term Treg-cell depletion led to a substantial increase in the frequencies of T cells in the tumors, attributed by both increased infiltration and proliferation of T cells in the Treg-cell-depleted tumors. We also demonstrate a selective increase of the chemokines CXCL9 and CXCL10 in Treg-cell-depleted tumors, which were accompanied by accumulation of CXCR3(+) T cells, and increased IFN-γ mRNA expression. In conclusion, Treg-cell depletion increases the accumulation of conventional T cells in intestinal tumors, and targeting Treg cells could be a possible anti-tumor immunotherapy, which not only affects T-cell effector functions, but also their recruitment to tumors.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?