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Trabecular bone loss in collagen antibody-induced arthritis

Journal article
Authors Louise Grahnemo
Annica Andersson
Merja Nurkkala Karlsson
Alexandra Stubelius
Marie K Lagerquist
Mattias Svensson
Claes Ohlsson
Hans Carlsten
Ulrika Islander
Published in Arthritis Research & Therapy
Volume 17
Issue 1
Pages art. no 189
ISSN 1478-6354
Publication year 2015
Published at Institute of Medicine, Department of Rheumatology and Inflammation Research
Centre for Bone and Arthritis Research
Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
Pages art. no 189
Language en
Links dx.doi.org/10.1186/s13075-015-0703-...
Keywords RHEUMATOID-ARTHRITIS, OSTEOCLAST DIFFERENTIATION, COMPLEMENT ACTIVATION, SYSTEMIC BONE, II COLLAGEN, DISEASE, MECHANISMS, MODEL, SEX, OSTEOPOROSIS, Rheumatology
Subject categories Rheumatology and Autoimmunity

Abstract

Introduction: Postmenopausal women with rheumatoid arthritis (RA) have increased risk of developing osteoporosis due to chronic inflammation and estrogen deprivation. Collagen antibody-induced arthritis (CAIA), an experimental polyarthritis model representing the effector phase of arthritis, is mainly mediated by the innate immune system. Compared to the widely used collagen-induced arthritis model, CAIA is conveniently short and can be used in C57BL/6 mice, enabling studies with knock-out mice. However, the impact on bone of the CAIA model in C57BL/6 mice has not previously been studied. Therefore, the aim of this study was to determine if CAIA can be used to study postmenopausal arthritis-induced osteoporosis. Methods: CAIA was induced by administration of collagen-type II antibodies and lipopolysaccharide to ovariectomized female C57BL/6J mice. Control mice received lipopolysaccharide, but no antibodies. Nine days later, femurs were collected for high-resolution micro-CT and histomorphometry. Serum was used to assess cartilage breakdown and levels of complement. Frequencies of immune cell subsets from bone marrow and lymph nodes were analyzed by flow cytometery. Results: Trabecular bone mass was decreased and associated with increased number of osteoclasts per bone surface in the CAIA model. Also, the frequency of interleukin-17(+) cells in lymph nodes was increased in CAIA. Conclusion: The present study show that CAIA, a short reproducible arthritis model that is compatible with C57BL/6 mice, is associated with increased number of osteoclasts and trabecular bone loss.

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