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Calibrators for Clinical Measurements of Phosphorylated H2AX in Patient Cells by Flow Cytometry

Journal article
Authors Aida Muslimovic
Pegah Johansson
Ulla Rüetschi
Ola Hammarsten
Published in The Open Clinical Chemistry Journal
Volume 5
Issue 1
Pages 33-39
ISSN 2588-7785
Publication year 2012
Published at Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Pages 33-39
Language en
Links dx.doi.org/10.2174/1874241601205010...
Keywords H2AX, flow cytometry
Subject categories Clinical Laboratory Medicine, Cancer and Oncology, Clinical Medicine

Abstract

Abstract: Agents that induce DNA double-strand breaks (DSBs), such as ionizing radiation, are frequently used in cancer therapy. H2AX is rapidly phosphorylated in response to DSBs and serves as a way to measure the extent of DSBs induced in patient cells. We have previously reported a flow cytometry-based method for measuring H2AX phosphorylation in pa-tient cells undergoing radiotherapy. To be able to implement measurement of H2AX phosphorylation in clinical practice, we have characterized calibrators for the flow cytometry analysis based on phosphopeptide-coated beads and fixed cells. The calibrator beads and fixed cells lost less than 11% of the signal after storage for 40 days under optimal conditions and were able to correct for day-to-day variation in method performance.

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