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Quantitative proteomics reveals regulatory differences in the chondrocyte secretome from human medial and lateral femoral condyles in osteoarthritic patients

Journal article
Authors Johan Stenberg
Ulla Rüetschi
Eva Skiöldebrand
Johan Kärrholm
Anders Lindahl
Published in Proteome Science
Volume 11
Issue 1
Pages UNSP 43
ISSN 1477-5956
Publication year 2013
Published at Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Institute of Clinical Sciences, Department of Orthopaedics
Pages UNSP 43
Language en
Links dx.doi.org/10.1186/1477-5956-11-43
Keywords Secretome, SILAC, Chondrocyte, Osteoarthritis, Proteomics, HUMAN ARTICULAR CHONDROCYTES, ENDOTHELIAL-CELL MIGRATION, PROTEIN, PHOSPHATASE 2A, LARGE GENE LISTS, SIGNALING PATHWAY, KNEE, OSTEOARTHRITIS, BIOCHEMICAL MARKER, SYNOVIAL-FLUIDS, CARTILAGE, IDENTIFICATION
Subject categories Orthopedics, Cell and Molecular Biology

Abstract

Background: Osteoarthritis (OA) is a destructive joint disease and there are no known biomarkers available for an early diagnosis. To identify potential disease biomarkers and gain further insight into the disease mechanisms of OA we applied quantitative proteomics with SILAC technology on the secretomes from chondrocytes of OA knees, designated as high Mankin (HM) scored secretome. A quantitative comparison was made between the secretomes of the medial and lateral femur condyle chondrocytes in the same knee since the medial femur condyle is usually more affected in OA than the lateral condyle, which was confirmed by Mankin scoring. The medial/lateral comparison was also made on the secretomes from chondrocytes taken from one individual with no clinically apparent joint-disease, designated as low Mankin (LM) scored secretome. Results: We identified 825 proteins in the HM secretome and 69 of these showed differential expression when comparing the medial and lateral femoral compartment. The LM scored femoral condyle showed early signs of OA in the medial compartment as assessed by Mankin score. We here report the identification and relative quantification of several proteins of interest for the OA disease mechanism e.g. CYTL1, DMD and STAB1 together with putative early disease markers e.g. TIMP1, PPP2CA and B2M. Conclusions: The present study reveals differences in protein abundance between medial/lateral femur condyles in OA patients. These regulatory differences expand the knowledge regarding OA disease markers and mechanisms.

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